ViraSafe™ Lentiviral Bicistronic Expression System (Puro), Ecotropic

Size

1 test kit

Product number

VPK-215-ECO

Price

1298.00€

ViraSafe™ Lentiviral Bicistronic Expression System (Puro), Ecotropic
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This reagent is purified

0.97

Species compatible to

Humans

The temperatureerature for storage is

-20ºC

We send this reagant on

Blue Ice

Use of this test kit or product

Genetics lab

Living modified organisme in this reagent or LMO

Not applicable

Type of reagent

Viral Expression

Molecular weight

in kilo Danlto or kDa

Antigen

a specific protein sequence

Reagent properties

Cloning capacity of expression vector = 7.8 kb

Buffer

The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,59

Protocol information

Complete Expression System contains packaging plasmids and expression vector

Related techniques

Viral Expression, Lentiviral Expression, Lentiviral Expression and Packaging, Lentiviral Expression Systems

Cellular use

Significantly reduced homology compared to other 3rd generation expression systems for added safety, without loss of titer

SDS or safty data sheet

(VPK-205 through VPK-222-xxx) GHS.pdf

Use of the reagent

Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe™ Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. Our Ecotropic Expression Systems assemble lentiviruses with an ecotropic envelope protein which will readily infect only mouse and rat cells via receptor-mediated binding, providing an additional level of safety. However, ecotropic viruses are not as stable upon freezing and will not survive ultracentrifugation procedures.